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KMID : 0811720210250020119
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Korean Journal of Physiology & Pharmacology
2021 Volume.25 No. 2 p.119 ~ p.129
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Centromere protein U enhances the progression of bladder cancer by promoting mitochondrial ribosomal protein s28 expression
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Liu Bei-Bei
Ma Tao
Sun Wei
Gao Wu-Yue
Liu Jian-Min
Li Li-Qiang
Li Wen-Yong
Wang Sheng
Guo Yuan-Yuan
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Abstract
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Bladder cancer is one of the most common types of cancer. Most gene mutations related to bladder cancer are dominantly acquired gene mutations and are not inherited. Previous comparative transcriptome analysis of urinary bladder cancer and control samples has revealed a set of genes that may play a role in tumor progression. Here we set out to investigate further the expression of two candidate genes, centromere protein U (CENPU) and mitochondrial ribosomal protein s28 (MRPS28) to better understand their role in bladder cancer pathogenesis. Our results confirmed that CENPU is up-regulated in human bladder cancer tissues at mRNA and protein levels. Gain-of-function and loss-of-function studies in T24 human urinary bladder cancer cell line revealed a hierarchical relationship between CENPU and MRPS28 in the regulation of cell viability, migration and invasion activity. CENPU expression was also up-regulated in in vivo nude mice xenograft model of bladder cancer and mice overexpressing CENPU had significantly higher tumor volume. In summary, our findings identify CENPU and MRPS28 in the molecular pathogenesis of bladder cancer and suggest that CENPU enhances the progression of bladder cancer by promoting MRPS28 expression.
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KEYWORD
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Bladder cancer, Cell, Centromere, Protein
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